Keson Trakunram
Prince of Songkla University, Thailand
Title: Up-regulation of serum mir-339-3p as a diagnostic Biomarker for non-small cell lung cancers
Biography
Biography: Keson Trakunram
Abstract
MicroRNAs (miRNAs), small non-coding RNA, play important role as negative
regulation of gene expression. Deregulation of miRNAs has been used for
predicting cancer progression in various cancers, including non-small cell lung cancer
(NSCLC). Therefore, searching for potential non-invasive biomarkers is need for rapid
diagnosis of lung cancer. This study aimed to evaluate whether serum miR-339-3p can
be a diagnostic biomarker for NSCLC. A retrospective study was designed. Serum was
collected from 151 suspected patients with lung cancer. All patients were diagnosed at
Songklanagarind Hospital (Hat Yai, Thailand), a university hospital in Southern Thailand,
between December 2017 and September 2018. Expression of miR-339-3p was determined
by quantitative real-time PCR (qRT-PCR) technique. Receiver operating characteristic
(ROC) curve and the area under the curve (AUC) were used to test the diagnostic
performance. Result showed that 66 patients were NSCLC and 85 patients were non-
NSCLC. The mean relative expression of serum miR-339-3p in NSCLC patients was
approximately two times higher compared with that of non-NSCLC patients with P-value
of 0.009. In addition, the ROC curve data indicated that this serum miR-339-3p can
distinguish the NSCLC patients from the non-NSCLC patients with the AUC of 0.624
(95% CI: 0.53-0.71). The sensitivity was 73% and the specificity was 45%. In conclusion,
serum miR-339-3p may serve as a circulating biomarker for diagnosis of lung cancer.