Man Hee Rhee
Kyungpook National University, South Korea
Title: Ginsenoside Rg3-enriched red ginseng extract potently suppress inflammation in RAW 264.7 cells
Biography
Biography: Man Hee Rhee
Abstract
The potential benefits of ginseng range from energizing the body to providing the longevity. Ginsenoside Rg3 (Rg3), one of the most effective ginseng saponins, has anti-inflammatory and anti-cancer effects. Numerous previous studies have unraveled the Rg3 Pharmacological effects. One recent study has also revealed its protective cardiovascular effects in hyper sensitive rats. Here we investigated the anti-inflammatory activity of Rg3 in vitro on murine macrophages RAW 264.7 cells. We found that Rg3 in dose dependent manner attenuate the expression of proinflammatory cytokines after LPS induction. The MTT assay for dose dependent concentrations of Rg3 show no cytotoxicity and Nitric oxide concentration show a decreasing trend with increase in dose. The mRNA expression of proinflammatory cytokines like IL-1β, IL-6, iNOS, COX-2 and TNF-α show the same trend like that of Nitric oxide production. The protein expression shown by Rg3-RGE clearly indicates that it signal transduces via MAPK and NF-κB pathways. More investigation into signal mediation revealed that Rg3-RGE mediates its antiinflammatory action via RXRα receptors. Moreover the our invivo data shows that Rg3-RGE very strongly protected the mice against LPS induced septic shock.